A limitation lifted: A conditional knockdown system reveals essential roles for Polo-like kinase and Aurora kinase 1 in Trypanosoma cruzi cell division.
Wiedeman J, Harrison R, Etheridge RD
Proc Natl Acad Sci U S A. 2025 Feb 25;122(8):e2416009122. doi: 10.1073/pnas.2416009122. Epub 2025 Feb 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233143 | pTMiniTrex-mCherry-Shark1 | Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette |
| 233144 | pTMiniTrex-mCherry-Shark2 | Serves as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassette |
| 233145 | pTMiniTrex-mCherry-Shark3 | Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette |