Digital and Tunable Genetically Encoded Tension Sensors Based on Engineered Coiled-Coils.
Liu S, Liu J, Foote A, Ogasawara H, Al Abdullatif S, Batista VS, Salaita K
Angew Chem Int Ed Engl. 2025 Jan 13:e202407359. doi: 10.1002/anie.202407359.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233226 | Vh-Clover-CC-S0-mRuby2-Vt | Vinculin FRET tension sensor with coiled-coil S0 domain |
| 233227 | Vh-Clover-CC-S2-mRuby2-Vt | Vinculin FRET tension sensor with coiled-coil S2 domain |
| 233228 | Vh-Clover-CC-S4-mRuby2-Vt | Vinculin FRET tension sensor with coiled-coil S4 domain |
| 233229 | Vh-Clover-CC-S7-mRuby2-Vt | Vinculin FRET tension sensor with coiled-coil S7 domain |
| 233230 | Vh-Clover-CC-S1-mRuby2-Vt | Vinculin FRET tension sensor with coiled-coil S1 domain |
| 233231 | Vh-Clover-CC-M2-mRuby2-Vt | Vinculin FRET tension sensor with coiled-coil M2 domain |
| 233232 | Vh-Clover-CC-Mad-mRuby2-Vt | Vinculin FRET tension sensor with coiled-coil Mad domain |
| 233233 | Vinculin-Clover-CC-S0-mRuby2 | Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control |
| 233239 | Vinculin-Clover-CC-Mad-mRuby2 | Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control |
| 233240 | pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt | Parallel coiled-coil with (GGS)3 linker to prone condensation |
| 233241 | pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt | Parallel coiled-coil with (GGS)12 linker to prevent condensation |
| 233243 | pET28a-AviTag-CC-S2 | Bacterial expression of Avi-CC-S2 |