A subcellular map of translational machinery composition and regulation at the single-molecule level.
Zhang Z, Xu A, Bai Y, Chen Y, Cates K, Kerr C, Bermudez A, Susanto TT, Wysong K, Garcia Marques FJ, Nolan GP, Pitteri S, Barna M
Science. 2025 Mar 7;387(6738):eadn2623. doi: 10.1126/science.adn2623. Epub 2025 Mar 7.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
235609 | PX459_L31_CRISPR_upstream_sgRNA | Encodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31 |
235610 | PX459_L31_CRISPR_downstream_sgRNA | Encodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31 |
235611 | pCAGEN_GFP | Encodes GFP. For use in transfections as a negative control without epitope tags. |
235612 | pCAGEN_N65_eMagBF_V5_NES | Encodes ALIBi N-terminal fragment, residues 1-65, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence. |
235613 | pCAGEN_NES_V5_eMagAF_C66 | Encodes ALIBi C-terminal fragment, residues 66-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence |
235614 | pCAGEN_N73_eMagBF_V5_NES | Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence. |
235615 | pCAGEN_NES_V5_eMagAF_C74 | Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence |
235616 | pCAGEN_N77_eMagBF_V5_NES | Encodes ALIBi N-terminal fragment, residues 1-77, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence. |
235617 | pCAGEN_NES_V5_eMagAF_C78 | Encodes ALIBi C-terminal fragment, residues 78-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence |
235618 | pCAGEN_N99_eMagBF_V5_NES | Encodes ALIBi N-terminal fragment, residues 1-99, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence. |
235619 | pCAGEN_NES_V5_eMagAF_C100 | Encodes ALIBi C-terminal fragment, residues 100-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence |
235620 | pCAGEN_N140_eMagBF_V5_NES | Encodes ALIBi N-terminal fragment, residues 1-140, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence. |
235621 | pCAGEN_NES_V5_eMagAF_C141 | Encodes ALIBi C-terminal fragment, residues 141-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence |
235622 | pCAGEN_N167_eMagBF_V5_NES | Encodes ALIBi N-terminal fragment, residues 1-167, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence. |
235623 | pCAGEN_NES_V5_eMagAF_C168 | Encodes ALIBi C-terminal fragment, residues 168-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence |
235624 | pCAGEN_N195_eMagBF_V5_NES | Encodes ALIBi N-terminal fragment, residues 1-195, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence. |
235625 | pCAGEN_NES_V5_eMagAF_C196 | Encodes ALIBi C-terminal fragment, residues 196-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence |
235626 | pCAGEN_N221_eMagBF_V5_NES | Encodes ALIBi N-terminal fragment, residues 1-221, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence. |
235627 | pCAGEN_NES_V5_eMagAF_C222 | Encodes ALIBi C-terminal fragment, residues 222-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence |
235628 | pCAGEN_N232_eMagBF_V5_NES | Encodes ALIBi N-terminal fragment, residues 1-232, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence. |
235629 | pCAGEN_NES_V5_eMagAF_C233 | Encodes ALIBi C-terminal fragment, residues 233-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence |
235630 | pCAGEN_N256_eMagBF_V5_NES | Encodes ALIBi N-terminal fragment, residues 1-256, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence. |
235631 | pCAGEN_NES_V5_eMagAF_C257 | Encodes ALIBi C-terminal fragment, residues 257-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence |
235632 | pCAGEN_NES_V5_GFP | Encodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control. |
235633 | pCAGEN_NES_V5_GFP_FTA | Encodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi. |
235634 | pCAGEN_NES_V5_BirA | Encodes full-length BirA fused to a nuclear export sequence and V5. Used as a positive control in ALIBi experiments |
235635 | pCAGEN_NES_V5_eMagBF_N73 | Encodes fusion protein of nuclear export sequence, V5, eMagBF (enhanced Magnet, basic, fast), ALIBi N-terminal fragment (residues 1-73) (order reversed) |
235636 | pCAGEN_C74_eMagAF_V5_NES | Encodes fusion protein of ALIBi C-terminal fragment (residues 74-321), eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence (order reversed) |
235637 | pCAGEN_NES_V5_eMagAF_C74_Sec61b | Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, Sec61b for targeting ALIBi to endoplasmic reticulum |
235638 | pCAGEN_NES_V5_eMagAF_C74_MAVS | Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, MAVS residues 500-540 for targeting to outer mitochondrial membrane |
235639 | pCAGEN_MyrPalm_NES_V5_eMagAF_C74 | Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, MyrPalm for targeting to plasma membrane |
235640 | pCAGEN_V5_eMagAF_C74_cMycNLS | Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus |
235641 | pCAGEN_V5_Fbl_eMagAF_C74 | Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, Fbl for targeting to nucleolus |
235642 | pCAGEN_N73_eMagBF_V5 | Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization. |
235643 | pCAGEN_Fbl_N73_eMagBF_V5 | Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, Fbl for targeting to nucleolus. |