N-terminal fragment shedding contributes to signaling of the full-length adhesion receptor ADGRL3.
Perry-Hauser NA, Du Rand JR, Lee KH, Shi L, Javitch JA
J Biol Chem. 2025 Jan 9:108174. doi: 10.1016/j.jbc.2025.108174.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233062 | pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3 | Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. |
| 233063 | pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G | Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. |
| 233064 | pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT | Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag. |
| 233065 | pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A | Expresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. |