Jonathan Ploski Lab: Ploski Lab Plasmids 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233025 | pAAV-EFS-CasRx-P2A-mCherry-pA | To Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A site |
| 233026 | pAAV-EFS-hfCas13d-P2A-mCherry-pA | To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site |
| 233027 | pAAV-EFS-DjCas13d-P2A-mCherry-pA | To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site |
| 233028 | pAAV-EFS-CasRx-HA-mCherry-pA | To Express HA tagged CasRX-mCherry fusion protein from the mammalian EFS promoter |
| 233029 | pAAV-EFS-hfCas13d-HA-mCherry-pA | To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter |
| 233030 | pAAV-EFS-DjCas13d-HA-mCherry-pA | To Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoter |
| 233031 | pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA | To express a DjCas13d-compatible gRNA |
| 233032 | pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa | To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome |
| 233033 | pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa | To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome |
| 233034 | pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa | To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome |
| 233035 | pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa | To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter |
| 233036 | pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa | To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter |
| 233037 | pAAV-EF1a-hfCas13d-pa | To express hfCas13d from a EF1a promoter |
| 233038 | pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA | To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter |
| 233039 | pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA | To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter |
| 233040 | pAAV-EF1a-mHFE(H67D)-IRES-GFP-WPRE-pA | To express mouse homeostatic iron regulator (HFE)(H67D) from an EF1a promoter. It also encodes GFP after an IRES sequence |
| 233041 | pAAV-EF1a-DIO-FLAG-mouse Beta Adrenergic Receptor (#1) | To Express a CRE dependent Flag tagged Mouse Beta Adrenergic Receptor from a EF1a promoter |
| 233042 | pLenti-CMV-eGFP-hTau E14-WPRE | To Express a GFP human Tau E14 fusion protein from a CMV promoter |
| 233043 | pLenti-CMV-eGFP-hTau P301L-WPRE | To Express a GFP human Tau P301L fusion protein from a CMV promoter |
| 233044 | pLenti-CMV-eGFP-hTau AP- WPRE | To Express a GFP human Tau AP fusion protein from a CMV promoter |
| 233045 | pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA | To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site. |
| 233046 | pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA | To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site. |
| 233047 | pLenti EFS-GFP-pa (opposite orientation)- TRE3g-Flag-GluN2B-WPRE | To express GFP from an EFS promoter in the 3'-5' direction and Flag-GluN2B from a TRE3g promoter in the 5'-3' direction |
| 233053 | pAAV-CMV-Flag-Rat Arc-Arc- Genomic 3'UTR | To express Flag-tagged Rat Arc from a CMV promoter. The Arc gene contains the Rat Arc native Genomic 3'UTR containing two introns |
| 233056 | pAAV-Rat SARE-Arc Min Promoter-Flag Arc-Arc Genomic 3'UTR | To express Flag-tagged Rat Arc from a Rat SARE-Arc minimal promoter. The Arc gene contains the Rat Arc native Genomic 3'UTR containing two introns |