CTCF-RNA interactions orchestrate cell-specific chromatin loop organization
Lucero K, Han S, Huang P, Qiu X, Mazzoni EO, Reinberg D
bioRxiv, March 19, 2025
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232926 | KL060 - pBlueScript II SK-mAID-EGFP CTCF donor | Donor plasmid for tagging mouse CTCF with mAID-eGFP at the N-terminus. Used with KL061 - PX330-sgRNA006_CTCF sgRNA. |
| 232928 | PX330-sgRNA006_CTCF | Expression vector for a sgRNA against the mouse CTCF locus and SpCas9. |
| 232929 | KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26 | Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA. |
| 232930 | KL094 - pTRE3G-Flag-Halo-CTCF_WT-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor | Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF WT, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection. |
| 232931 | KL095 - pTRE3G-Flag-Halo-CTCF_ZF1_mutant-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor | Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF ZF1 mutant, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection. |
| 232932 | PX459-sgRNA047_Podxl | Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene. |
| 232934 | PX459-sgRNA048_Grb10 | Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene. |
| 232935 | KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA | Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA. |
| 232936 | KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor | Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA. |
| 233090 | PX458-eBFP2-sgRNA_CTCF_ZF1 | Expression vector for a sgRNA against the mouse CTCF ZF1 region and SpCas9-T2A-eBFP2. |