Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles.
Halegua T, Risson V, Carras J, Rouyer M, Coudert L, Jacquier A, Schaeffer L, Ohlmann T, Mangeot PE
Nat Commun. 2025 Jan 4;16(1):397. doi: 10.1038/s41467-024-55604-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232920 | pp-CBBC-Fluc | Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells. |
| 232921 | GAG-PEmax V7 | Optimized construct for the expression of PEmax fused to GAG from fMLV. This plasmid is involved in the production of NanoScribes |