CnRed: Efficient, Marker-free Genome Engineering of Cupriavidus necator H16 by Adapted Lambda Red Recombineering.
Arhar S, Pirchner J, Stolterfoht-Stock H, Reicher K, Kourist R, Emmerstorfer-Augustin A
ACS Synth Biol. 2025 Feb 24. doi: 10.1021/acssynbio.4c00757.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232907 | pCnCre | Electroporation vector for expression of Cre recombinase in Cuprividus necator |
| 232908 | pCnRed | Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator. |
| 232909 | pHRep-HKH1 | Plasmid for disruption of the phaC1 locus of Cupriavidus necator via homologous recombination |
| 232910 | pHInt-AppA-KanR-loxP | Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator. |