Development of a genetically-encoded in vivo computing platform to play the game of Tic-Tac-Toe
Kushwaha M, Singh V, Jaramillo A
Zenodo (2017) Conference: SB7.0: The Seventh International Meeting on Synthetic Biology.
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231410 | pSEVA19_sgRNA-C0_M13ps | M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.) |
| 231411 | pSEVA19_sgRNA-C1_M13ps | M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.) |
| 231412 | pSEVA26_sgRNA-2_M13ps | M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.) |
| 231414 | pSEVA26_sgRNA-A1_M13ps | M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.) |
| 231417 | pSEVA47_dCas9-GFP_NOT_iA | This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.) |
| 231420 | pSEVA47_Helper | M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ296.) |