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Conserved role of spike S2 domain N-glycosylation across betacoronaviruses

Yang Q, Kelkar A, Manicassamy B, Neelamegham S
npj Viruses. 2025 Jan; 3(4). doi: 10.1038/s44298-024-00085-7. (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
231904LVDP 2P.2For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPs
231905LVDP 2P.2.EGFPFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPs
231913SARS-CoV-2 spike G1 mutantFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098 by implementing Asn-to-Gln mutation
231914SARS-CoV-2 spike G12 mutantFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134 by implementing Asn-to-Gln mutation
231915SARS-CoV-2 spike G13 mutantFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158 by implementing Asn-to-Gln mutation
231916SARS-CoV-2 spike G14 mutantFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173 by implementing Asn-to-Gln mutation
231917SARS-CoV-2 spike G15 mutantFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1194 by implementing Asn-to-Gln mutation
231918SARS-CoV-2 spike G145 mutantFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173, N1194 by implementing Asn-to-Gln mutation
231919SARS-CoV-2 spike G1345 mutantFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158, N1173, N1194 by implementing Asn-to-Gln mutation
231920SARS-CoV-2 spike G12345 mutantFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134, N1158, N1173, N1194 by implementing Asn-to-Gln mutation
231921SARS-CoV spike all5KO mutantFor expression of SARS-CoV spike protein with glycan deletion at N1080, N1116, N1140, N1155, N1176 by implementing Asn-to-Gln mutation
231923MERS-CoV spike last4KO mutantFor expression of MERS-CoV spike protein with glycan deletion at N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutation
231924MERS-CoV spike all7KO mutantFor expression of MERS-CoV spike protein with glycan deletion at N1176, N1213, N1225, N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutation

Antibodies from Article