Karsten Rippe Lab: HP1 binding creates a local barrier against transcription activation and persists during chromatin decondensation
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232630 | pdCas9-GFP-p65 | p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer |
| 232631 | pdCas9-tdTomato | catalytically inactive dCas9 for localization; labeled with tdTomato |
| 232632 | pdCas9-tdTomato-VPR | VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer |
| 232633 | rTetR-GFP-p65 | p65 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition |
| 232636 | HaloTag-LacI #1691 | lac repressor (LacI) which binds lacO sites; labeled with HaloTag |
| 232637 | HaloTag-LacI-mHP1a | lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alpha |
| 232638 | HaloTag-LacI-KRAB | lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with Krüppel-associated box (KRAB) repressor |