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TWISP: a transgenic worm for interrogating signal propagation in Caenorhabditis elegans.

Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
124343pAS1-rab-3P::his-24::tagBFPNuclear Localized Pan-Neuronal tagBFP
124351pAS1-rab-3P::his-24::GCaMP6sNuclear Localized Pan-Neuronal GCaMP6s
232601pAS3-rab-3P::AI::ChR2(H134R)Pan-neuronal expression of opsin 'ChR2(H134R)', regulated by rab-3 promoter and unc-54 3' UTR
232602pAS3-rab-3P::AI::VoltronPan-neuronal expression of voltage sensor 'Voltron', regulated by rab-3 promoter and unc-54 3' UTR
232603pAS3-rab-3P::CHRIMSON_CEPan-neuronal expression of opsin 'Chrimson', regulated by rab-3 promoter and unc-54 3' UTR
232604pAS3-rab-3P::AI::TsChRPan-neuronal expression of opsin 'TsChR', regulated by rab-3 promoter and unc-54 3' UTR
232605pAS3-rab-3P::AI::gur-3GPan-neuronal expression of 'GUR-3' using a genomic fragment, regulated by rab-3 promoter and unc-54 3' UTR
232606pAS3-rab-3P::AI::lite-1GPan-neuronal expression of 'LITE-1' using a genomic fragment, regulated by rab-3 promoter and unc-54 3' UTR
232607pAS3-rab-3P::AI::QF+hGR::SL2::tagBFPPan-neuronal expression of chimeric protein 'QF+hGR' and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR
232608pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFPPan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR
232609pAS3-rab-3P::AI::prdx-2G::SL2::his-24::tagRFPPan-neuronal expression of 'PRDX-2' using a genomic fragment and fluorophore 'tagRFP', regulated by rab-3 promoter and unc-54 3' UTR
232610pAS3-rab-3P::AI::lite-1G::SL2::his-24::tagBFPPan-neuronal expression of 'LITE-1' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR
232611pAS3-rab-3P::AI::QF+hGRPan-neuronal expression of chimeric protein 'QF+hGR', regulated by rab-3 promoter and unc-54 3' UTR
232612pAS3-5xQUAS+Δpes-10P::AI::eTsChRExpression of opsin 'eTsChR' on activation via 'QF+hGR' chimeric protein
232613pAS3-5xQUAS::Δpes-10P::AI::gur-3GExpression of 'GUR-3' on activation via 'QF+hGR' chimeric protein
232614pAS3-5xQUAS::Δpes-10P::AI::prdx-2GExpression of 'PRDX-2' on activation via 'QF+hGR' chimeric protein

Antibodies from Article