A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates.
Mukhopadhyay U, Levantovsky S, Carusone TM, Gharbi S, Stein F, Behrends C, Bhogaraju S
Sci Adv. 2024 Aug 9;10(32):eadp3000. doi: 10.1126/sciadv.adp3000. Epub 2024 Aug 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232577 | pcDNA5-AP-Ub | Express Ubiquitin tagged with an Acceptor Peptide (AP) |
| 232581 | pcDNA5-AP-Ub-dGG | Express Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycines |
| 232582 | pcDNA5-(-2)AP-Ub | Express Ubiquitin tagged with a modified Acceptor Peptide (AP) |
| 232583 | pcDNA5-AP(-3)-Ub | Express Ubiquitin tagged with a modified Acceptor Peptide (AP) |
| 232584 | pEHAC1-HA-BirAonly | Express HA-BirA |
| 232586 | pEHAC1-HA-BirA-GSGS-Traf6 | Express BirA tagged to the N-terminus of TRAF6 with a GSGS linker between BirA and TRAF6. |
| 232587 | pEHAC1-CHIP-BirA-HA | Express BirA tagged to the C-terminus of CHIP. |
| 232588 | pEHAC1-CHIP-GSGS-BirA-HA | Express BirA tagged to the C-terminus of CHIP with a GSGS linker between BirA and CHIP. |