Enhancement of erythropoietic output by Cas9-mediated insertion of a natural variant in haematopoietic stem and progenitor cells.
Luna SE, Camarena J, Hampton JP, Majeti KR, Charlesworth CT, Soupene E, Selvaraj S, Jia K, Sheehan VA, Cromer MK, Porteus MH
Nat Biomed Eng. 2024 Dec;8(12):1540-1552. doi: 10.1038/s41551-024-01222-6. Epub 2024 Jun 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232404 | MKC89_CCR5(UbC-tEPOR-2A-YFP) | AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs. |
| 232406 | MKC91_tEPOR(UbC-GFP-BGH) | AAV production plasmid for tEPOR-UbC-GFP-BGH vector from Fig. 1 that mediates HDR at EPOR locus using EPOR-sg1 gRNA. Repair vector introduces premature stop codon into EPOR locus. |