Chenggang Wu Lab: Development of a Conditional Plasmid for Gene Deletion in Non-Model Fusobacterium nucleatum strains

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
232318	pCWU50	A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch
232319	pZP05	pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum
232320	pZP06	pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.