Chenggang Wu Lab: Development of a Conditional Plasmid for Gene Deletion in Non-Model Fusobacterium nucleatum strains
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232318 | pCWU50 | A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch |
| 232319 | pZP05 | pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum |
| 232320 | pZP06 | pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E. |
| 232321 | pZP06B | Make sacB a counterselection marker to replace galK |
| 232322 | pZP06C | pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence. |