Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo
Smith ER, Turner ED, Abdelhamid MAS, Craggs TD, Twelvetrees AE
bioRxiv 2024.12.20.629623
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200786 | pCLAP | Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein. |
| 200787 | pCDNA3.1-CLIPf | For the mammalian expression of CLIPf-HisTag-FLAG fusion protein. |
| 200788 | pCDNA3.1-SNAPf | For the mammalian expression of SNAPf-HisTag-FLAG fusion protein. |
| 200789 | CLIPf-KIF5A-SNAPf-HisTag-FLAG | Mammalian expression of CLIPf-KIF5A-SNAPf-HisTag-FLAG |
| 200790 | CLIPf-KIF5A | Mammalian expression of CLIPf-KIF5A |
| 200791 | KIF5A-SNAPf-HisTag-FLAG | Mammalian expression of KIF5A-SNAPf-HisTag-FLAG |
| 200792 | CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG | Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG |
| 200793 | CLIPf-KIF5A(1-915; Δ505-610)-SNAPf-HisTag-FLAG | Mammalian expression of CLIPf-KIF5A(1-915; Δ505-610)-SNAPf-HisTag-FLAG |
| 200798 | CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG | Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG |
| 200799 | CLIPf-KIF5B-SNAPf-HisTag-FLAG | Mammalian expression of CLIPf-KIF5B-SNAPf-HisTag-FLAG |
| 219681 | K560-Halo-Strep | Mammalian expression of KIF5B(1-560)-HaloTag-Strep |
| 219682 | Strep-Halo | Mammalian expression of Strep-HaloTag fusion protein |
| 219683 | CLIPf-KIF5A(1-573) | Mammalian expression of CLIPf-KIF5A(1-573) |