Cas9-mediated gene-editing frequency in microalgae is doubled by harnessing the interaction between importin alpha and phytopathogenic NLSs.
Le TT, Choi HI, Kim JW, Yun JH, Lee YH, Jeon EJ, Kwon KK, Cho DH, Choi DY, Park SB, Yoon HR, Lee J, Sim EJ, Lee YJ, Kim HS
Proc Natl Acad Sci U S A. 2025 Mar 11;122(10):e2415072122. doi: 10.1073/pnas.2415072122. Epub 2025 Mar 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232296 | pET-Octo_VirD2_NLS-Cas9-6xHis | Expresses Cas9 protein fused with octopine-type VirD2-originating NLS |