RLIM-specific activity reporters define variant pathogenicity in Tonne-Kalscheuer syndrome.
Bandi V, Rennie M, Koch I, Gill P, Pacheco OD, Berg AD, Cui H, Ward DI, Bustos F
HGG Adv. 2024 Oct 31;6(1):100378. doi: 10.1016/j.xhgg.2024.100378.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232239 | pCAGGS puro GFP-REX1-IRES-mCherry | Mammalian expression of REX1-GFP-IRES-mCherry |
| 232240 | pCAGGS puro GFP-REX1[degron]-IRES-mCherry | Mammalian expression of REX1[degron]-GFP-IRES-mCherry |
| 232241 | pGEX6P1 human RLIM N581K | Protein expression of GST-human RLIM N581K |
| 232242 | pCAGGS puro human RLIM N581K | Mammalian expression of Human RLIM N581K |
| 232243 | pCAGGS puro human-GFP-RLIM-IRES-mCherry | Mammalian expression of RLIM-GFP-IRES-mCherry |
| 232244 | pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry | Mammalian expression of N581K RLIM-GFP-IRES-mCherry |
| 232245 | pCAGGS puro -GFP-I77P I78P REX1-IRES-mCherry | Mammalian expression of I77P I78P REX1-GFP-IRES-mCherry |
| 232246 | pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry | Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry |
| 232247 | pCAGGS puro-GFP-BamHI-IRES-mCherry | AcGFP1-BamHI-IRES-mCherry empty backbone |