Activation of the imprinted Prader-Willi syndrome locus by CRISPR-based epigenome editing.
Rohm D, Black JB, McCutcheon SR, Barrera A, Berry SS, Morone DJ, Nuttle X, de Esch CE, Tai DJC, Talkowski ME, Iglesias N, Gersbach CA
Cell Genom. 2025 Feb 12;5(2):100770. doi: 10.1016/j.xgen.2025.100770.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232178 | pLV-UbC-Tet1c-dCas9 | Lentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cells |
| 232179 | pLV-UbC-VP64-dCas9-VP64 | Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells |
| 232180 | pLV-UbC-Tet1v4-dCas9 | Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells |
| 232183 | pLV-U6-gRNA scaffold-UbC-mCherry-PuroR | Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry |