Zebrafish are resilient to the loss of major diacylglycerol acyltransferase enzymes.
Wilson MH, Hensley MR, Shen MC, Lu HY, Quinlivan VH, Busch-Nentwich EM, Rawls JF, Farber SA
J Biol Chem. 2024 Nov 6;300(12):107973. doi: 10.1016/j.jbc.2024.107973.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232134 | pCRII-dgat2 in situ probe | zebrafish dgat2 anti-sense in situ probe, linearize with BamHI, T7 polymerase |
| 232135 | pcRII-mogat3a in situ probe | zebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymerase |
| 232136 | pCRII-mogat3b in situ probe | zebrafish mogat3b anti-sense in situ probe, linearize with BamHI, T7 polymerase |
| 232137 | pCRII-TOPO-dgat1a in situ probe | zebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymerase |
| 232138 | pCRII-TOPO-dgat1b in situ probe | zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase |
| 232139 | pCRII-TOPO-mogat2 in situ probe | zebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymerase |
| 232140 | pDEST-CMV-EGFPCAAX | Expresses EGFP with a CAAX motif under control of a CMV promoter |
| 232141 | pDEST-CMV-zdgat2FLAG-SV40 | Expresses zebrafish dgat2 with a C-terminal 3xFLAG tag under control of the CMV promoter |
| 232142 | pME-zdgat2-CDS-FLAG | Gateway middle entry vector containing zebrafish dgat2 with a C-terminal 3xFLAG tag |
| 232143 | pcDNA3.1+ dgat1a-FLAG | Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter |
| 232145 | pcDNA3.1+ mogat3b-FLAG | Expresses zebrafish mogat3b with a C-terminal FLAG tag under control of the CMV promoter |