Structural studies identify angiotensin II receptor blocker-like compounds as branched-chain ketoacid dehydrogenase kinase inhibitors.
Liu S, Kormos BL, Knafels JD, Sahasrabudhe PV, Rosado A, Sommese RF, Reyes AR, Ward J, Roth Flach RJ, Wang X, Buzon LM, Reese MR, Bhattacharya SK, Omoto K, Filipski KJ
J Biol Chem. 2023 Mar;299(3):102959. doi: 10.1016/j.jbc.2023.102959. Epub 2023 Jan 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232116 | pET28 His-MBP-TEV-BAP-Human BCKDK | For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag. |
| 232117 | pET28 E2 LBD-Tev-E1a-His | For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag. |
| 232118 | pETDuet-1 Flag-LplA | For bacterial expression of Flag-tagged E. coli LplA. |
| 232119 | pET28 MBP-TEV-Rat BCKDK-His | For bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag. |