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Improved vectors for retron-mediated CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.

Stuecker TN, Hood SE, Pineda JM, Lenaduwe S, Winter J, Sadhu MJ, Lewis JA
bioRxiv [Preprint]. 2024 Aug 7:2024.08.06.606807. doi: 10.1101/2024.08.06.606807. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
232092pCas9-EcRT-GAL-KanYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
232096pCas9-EcRT-Z3-KanYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

Antibodies from Article