Interleukin-1alpha release during necrotic-like cell death generates myeloid-driven immunosuppression that restricts anti-tumor immunity.
Hanggi K, Li J, Gangadharan A, Liu X, Celias DP, Osunmakinde O, Keske A, Davis J, Ahmad F, Giron A, Anadon CM, Gardner A, DeNardo DG, Shaw TI, Beg AA, Yu X, Ruffell B
Cancer Cell. 2024 Nov 13:S1535-6108(24)00402-1. doi: 10.1016/j.ccell.2024.10.014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231971 | pLVX-DmrB-Casp8-2A-mCherry-puro | Tet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosis |
| 231972 | pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro | Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis |
| 231973 | pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro | Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis |
| 231974 | pLVX MLKL(wt)--2A-mCherry-puro | Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression |
| 231975 | pLVX MLKL(S345D)-2A-mCherry-puro | Tet inducible expression of DmrB-Mlkl with mCherry to induce phosphomimetic MLKL S345D necroptosis |
| 231976 | pLVX MLKL(1-180)-2A-mCherry-puro | Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis |
| 231977 | pLenti-multi-CRISPR-sgCasp8_#1-puro | Knockout mouse Casp8 |
| 231978 | pLenti-multi-CRISPR-sgCasp8_#2-puro | Knockout mouse Casp8 |
| 231979 | pLenti-multi-CRISPR-sgMlkl_#1-puro | Knockout mouse Mlkl |
| 231980 | pLenti-multi-CRISPR-sgMlkl_#2-puro | Knockout mouse Mlkl |
| 231981 | pLenti-multi-CRISPR-sgRipk3_#1-puro | Knockout mouse Ripk3 |
| 231982 | pLenti-multi-CRISPR-sgRipk3_#2-puro | Knockout mouse Ripk3 |
| 231983 | pLenti-multi-CRISPR-sgRipk1_#2-puro | Knockout mouse Ripk1 |
| 231984 | pLenti-multi-CRISPR-sgRipk1_#1-puro | Knockout mouse Ripk1 |
| 231985 | pHIV-EF1a-2A-ZsGreen | Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen |
| 231986 | pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen | Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen |
| 231987 | pHIV-EF1a-Il1a-ZsGreen | Express IL-1alpha-ZsGreen fusion protein |
| 231988 | pLenti-CRISPRv2-sgNTC13-hygro | Knockout non-targeting control |
| 231989 | pLenti-CRISPRv2-sgNTC49-puro | Knockout non-targeting control |
| 231990 | pLenti-CRISPRv2-sgIl1r1_#2-hygro | Knockout mouse Il1r1 |