Tuning the tropism and infectivity of SARS-CoV-2 virus-like particles for mRNA delivery.
Yang Q, Davidson BA, Pajic P, Chen X, Gokcumen O, Gao M, Neelamegham S
Nucleic Acids Res. 2025 Feb 27;53(5):gkaf133. doi: 10.1093/nar/gkaf133.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231903 | pcDNA3.1 N-T2A-M-IRES-E | For the production of SARS-CoV-2 virus-like particles (VLPs) in the '3-plasmid' system |
| 231906 | Const.2 EGFP-P2A-dTomato-PS9 | For packaging EGFP and dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) |
| 231907 | Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9 | For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs) |
| 231908 | Const.4 EGFP-IRES-Luc-PS9 | For packaging EGFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs) |
| 231909 | Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9 | For packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs) |
| 231910 | Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9 | For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs) |
| 231911 | pcDNA3.1 Cas9-P2A-dTomato-PS9 | For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal |
| 231912 | pcDNA3.1 Cas9-P2A-dTomato-T20 | For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal |
| 231925 | pLKO.1 EF1a-EGFP | 3rd generation lentiviral transfer plasmid with EGFP reporter gene |