Reconciling competing models on the roles of condensates and soluble complexes in transcription factor function
Bremer A, Lang WH, Kempen RP, Sweta K, Taylor AB, Borgia MB, Ansari AZ, Mittag T
bioRxiv 2024.11.21.624739
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231855 | pDest17_FL Gcn4 | Bacterial expression of N-terminally 6His tagged FL Gcn4 |
| 231856 | pDest17_Gcn4 WT 44mer | Bacterial expression of N-terminally 6His tagged Gcn4 WT 44mer |
| 231857 | pDest17_Gcn4 ILVtoED | Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED |
| 231858 | pDest17_Gcn4 ILVtoED solvvol | Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol |
| 231859 | pDest17_Gcn4 ILVtoAS | Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS |
| 231860 | pDest17_Gcn4 Swap3 | Bacterial expression of N-terminally 6His tagged Gcn4 Swap3 |
| 231861 | pDest17_Gcn4 SATtoG KtoR | Bacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoR |
| 231862 | pDest17_Gcn4 W120A | Bacterial expression of N-terminally 6His tagged Gcn4 W120A |
| 231863 | pDest17_Gcn4 E3 | Bacterial expression of N-terminally 6His tagged Gcn4 E3 |
| 231864 | pDest17_Gcn4 F124I | Bacterial expression of N-terminally 6His tagged Gcn4 F124I |
| 231865 | pDest17_Gcn4 Swap2 | Bacterial expression of N-terminally 6His tagged Gcn4 Swap2 |
| 231866 | pDest17_Gcn4 K5 | Bacterial expression of N-terminally 6His tagged Gcn4 K5 |
| 231867 | pDest17_Gcn4 9acidA | Bacterial expression of N-terminally 6His tagged Gcn4 9acidA |
| 231868 | pDest17_Gcn4 E2K2 | Bacterial expression of N-terminally 6His tagged Gcn4 E2K2 |
| 231869 | pDest17_Gcn4 L123A+ | Bacterial expression of N-terminally 6His tagged Gcn4 L123A+ |
| 231870 | pDest17_Gcn4 R2 | Bacterial expression of N-terminally 6His tagged Gcn4 R2 |
| 231871 | pDest17_Gcn4 ILVtoED W | Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W |
| 231872 | pDest17_Gcn4 P+ | Bacterial expression of N-terminally 6His tagged Gcn4 P+ |
| 231873 | pDest17_Gcn4 L113A+ | Bacterial expression of N-terminally 6His tagged Gcn4 L113A+ |
| 231874 | pDest17_Gcn4 R3 | Bacterial expression of N-terminally 6His tagged Gcn4 R3 |
| 231875 | pDest17_Gcn4 F108A+ | Bacterial expression of N-terminally 6His tagged Gcn4 F108A+ |
| 231876 | pDest17_Gcn4 R3 W | Bacterial expression of N-terminally 6His tagged Gcn4 R3 W |
| 231877 | pDest17_Gcn4 solvvol | Bacterial expression of N-terminally 6His tagged Gcn4 solvvol |
| 231878 | pDest17_Gcn4 solvvol W | Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W |
| 231879 | pDest17_Gcn4 Swap1 | Bacterial expression of N-terminally 6His tagged Gcn4 Swap1 |
| 231880 | pDest17_Gcn4 S/ArotoL | Bacterial expression of N-terminally 6His tagged Gcn4 SArotoL |
| 231881 | pDest17_Gcn4 WTSLFD | Bacterial expression of N-terminally 6His tagged Gcn4 WTSLFD |
| 231882 | pDest17_Gcn4 StoL | Bacterial expression of N-terminally 6His tagged Gcn4 StoL |
| 231883 | pDest17_Gcn4 Aro2+ | Bacterial expression of N-terminally 6His tagged Gcn4 Aro2+ |
| 231884 | pDest17_Gcn4 Aro3+ | Bacterial expression of N-terminally 6His tagged Gcn4 Aro3+ |
| 231885 | pDest17_Gcn4 WW+ | Bacterial expression of N-terminally 6His tagged Gcn4 WW+ |
| 231886 | pDest17_DBD | Bacterial expression of N-terminally 6His tagged Gcn4 DBD |
| 231887 | pDest17_Med15 | Bacterial expression of N-terminally 6His tagged Gcn4 Med15 |
| 231888 | pDest17_Gcn4 WT 30mer | Bacterial expression of N-terminally 6His tagged Gcn4 WT 30mer |
| 232955 | pAG415Gal_FL Gcn4 | Galactose iduced expression of full-length Gcn4 in yeast |
| 232956 | pAG415Gal_Gcn4 WT 44mer | Galactose iduced expression of Gcn4 WT 44mer in yeast |
| 232957 | pAG415Gal_Gcn4 ILVtoED | Galactose iduced expression of Gcn4 ILVtoED in yeast |
| 232958 | pAG415Gal_Gcn4 ILVtoED solvvol | Galactose iduced expression of Gcn4 LVtoED solvvol in yeast |
| 232959 | pAG415Gal_Gcn4 ILVtoAS | Galactose iduced expression of Gcn4 ILVtoAS in yeast |
| 232960 | pAG415Gal_Gcn4 Swap3 | Galactose iduced expression of Gcn4 k0.47 in yeast |
| 232961 | pAG415Gal_Gcn4 SATtoG KtoR | Galactose iduced expression of Gcn4 ATtoG KtoR in yeast |
| 232962 | pAG415Gal_Gcn4 W120A | Galactose iduced expression of Gcn4 W120A in yeast |
| 232963 | pAG415Gal_Gcn4 E+ | Galactose iduced expression of Gcn4 E+in yeast |
| 232964 | pAG415Gal_Gcn4 F124I | Galactose iduced expression of Gcn4 F124I in yeast |
| 232965 | pAG415Gal_Gcn4 Swap2 | Galactose iduced expression of Gcn4 k0.19 in yeast |
| 232966 | pAG415Gal_Gcn4 K5 | Galactose iduced expression of Gcn4 K+ in yeast |
| 232967 | pAG415Gal_Gcn4 9acidA | Galactose iduced expression of Gcn4 9acidA in yeast |
| 232968 | pAG415Gal_Gcn4 E2K2 | Galactose iduced expression of Gcn4 EK+ in yeast |
| 232969 | pAG415Gal_Gcn4 L123A | Galactose iduced expression of Gcn4 L123A in yeast |
| 232970 | pAG415Gal_Gcn4 R2 | Galactose iduced expression of Gcn4 R+ in yeast |
| 232971 | pAG415Gal_Gcn4 P+ | Galactose iduced expression of Gcn4 Proline in yeast |
| 232972 | ppAG415Gal_Gcn4 L113A+ | Galactose iduced expression of Gcn4 L113A in yeast |
| 232973 | pAG415Gal_Gcn4 R3 | Galactose iduced expression of Gcn4 R++ in yeast |
| 232974 | pAG415Gal_Gcn4 F108A+ | Galactose iduced expression of Gcn4 F108A in yeast |
| 232975 | pAG415Gal_Gcn4 R3W | Galactose iduced expression of Gcn4 R++ W+ in yeast |
| 232976 | pAG415Gal_Gcn4 solvvol | Galactose iduced expression of Gcn4 solvvol in yeast |
| 232977 | pAG415Gal_Gcn4 solvvol W | Galactose iduced expression of Gcn4 solvvol W+ in yeast |
| 232979 | pAG415Gal_Gcn4 S/ArotoL | Galactose iduced expression of Gcn4 S/ArotoL in yeast |
| 232980 | pAG415Gal_Gcn4 WTSLFD | Galactose iduced expression of Gcn4 WTSLFD+ in yeast |
| 232981 | pAG415Gal_Gcn4 StoL | Galactose iduced expression of Gcn4 StoL in yeast |
| 232982 | pAG415Gal_Gcn4 Aro2+ | Galactose iduced expression of Gcn4 Aro2+ in yeast |
| 232983 | pAG415Gal_Gcn4 Aro3+ | Galactose iduced expression of Gcn4 Aro3+ in yeast |
| 232984 | pAG415Gal_Gcn4 WW+ | Galactose iduced expression of Gcn4 WW+ in yeast |
| 232985 | pAG415Gal_DBD | Galactose iduced expression of Gcn4 DBD in yeast |
| 232986 | pAG415Gal_Gcn4 WT 30mer | Galactose iduced expression of Gcn4 WT 30mer in yeast |
| 232987 | pAG415Gal_DBDHA | Galactose iduced expression of Gcn4 DBDAHA in yeast |
| 232988 | pAG415Gal_Gcn4 WT 44merHA | Galactose iduced expression of Gcn4 WT 44merHA in yeast |
| 232989 | pAG415Gal_Gcn4 Aro2+HA | Galactose iduced expression of Gcn4 Aro2+HA in yeast |
| 232990 | pAG415Gal_Gcn4 Aro3+HA | Galactose iduced expression of Gcn4 Aro3+HA in yeast |
| 232991 | pAG415Gal_Gcn4 WW+HA | Galactose iduced expression of Gcn4 WW+HA in yeast |
| 232992 | pAG415Gal_Gcn4 W120AHA | Galactose iduced expression of Gcn4 W120AHA in yeast |
| 232993 | pAG415Gal_Gcn4 F124IHA | Galactose iduced expression of Gcn4 F124IHA in yeast |
| 232994 | pAG415Gal_Gcn4 SATtoG KtoRHA | Galactose iduced expression of Gcn4 SATtoG KtoRHAin yeast |
| 232995 | pAG415Gal_Gcn4 ILVtoED WHA | Galactose iduced expression of Gcn4 ILVtoED W+HAin yeast |
| 232996 | pAG415Gal_Gcn4 E3HA | Galactose iduced expression of Gcn4 E+HA in yeast |
| 232997 | pAG415Gal_Gcn4 9acidAHA | Galactose iduced expression of Gcn4 9acidAHA in yeast |
| 232998 | pAG415Gal_Gcn4 Swap3HA | Galactose iduced expression of Gcn4 k0.47HA in yeast |
| 232999 | pAG415Gal_DBDGFP | Galactose iduced expression of Gcn4 DBDGFP in yeast |
| 233000 | pAG415Gal_Gcn4 WT 44merGFP | Galactose iduced expression of Gcn4 WT 44merGFP in yeast |
| 233001 | pAG415Gal_Gcn4 Aro2+GFP | Galactose iduced expression of Gcn4 Aro2+GFP in yeast |
| 233002 | pAG415Gal_Gcn4 Aro3+GFP | Galactose iduced expression of Gcn4 Aro3+GFP in yeast |
| 233003 | pAG415Gal_Gcn4 WW+GFP | Galactose iduced expression of Gcn4 WW+GFP in yeast |
| 233004 | pAG415Gal_Gcn4 W120AGFP | Galactose iduced expression of Gcn4 120AGFP in yeast |
| 233005 | pAG415Gal_Gcn4 F124IGFP | Galactose iduced expression of Gcn4 F124IGFP in yeast |
| 233006 | pAG415Gal_Gcn4 SATtoG KtoRGFP | Galactose iduced expression of Gcn4 SATtoG KtoRGFP in yeast |
| 233007 | pAG415Gal_Gcn4 ILVtoED WGFP | Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast |
| 233008 | pAG415Gal_Gcn4 E3GFP | Galactose iduced expression of Gcn4 E+GFP in yeast |
| 233009 | pAG415Gal_Gcn4 9acidAGFP | Galactose iduced expression of Gcn4 9acidAGFP in yeast |
| 233010 | pAG415Gal_Gcn4 Swap3GFP | Galactose iduced expression of Gcn4 k0.47GFP in yeast |