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Fine Tuning Genetic Circuits via Host Context and RBS Modulation

Chan DTC, Winter L, Bjerg J, Krsmanovic S, Baldwin GS, Bernstein HC
bioRxiv 2024.07.20.604438 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
231615pVCS1Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS1 at R2 site.
231616pVCS2Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS2 at R2 site.
231617pVCS3Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS3 at R2 site.
231618pVCS4Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS1 at R2 site.
231619pVCS5Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS2 at R2 site.
231620pVCS6Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS3 at R2 site.
231622pVCS8Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS2 at R2 site.
231623pVCS9Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS3 at R2 site.

Antibodies from Article