Long-term imaging of individual ribosomes reveals ribosome cooperativity in mRNA translation.
Madern MF, Yang S, Witteveen O, Segeren HA, Bauer M, Tanenbaum ME
Cell. 2025 Jan 23:S0092-8674(25)00045-5. doi: 10.1016/j.cell.2025.01.016.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231591 | pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag | Express circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter |
| 231592 | pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Xbp1(S255A) | Express circular RNA translation reporter with 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter |
| 231593 | pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Pseudoknot | Express circular RNA translation reporter with 2xALFATag-10xSunTag-Pseudoknot under H1 promoter |
| 231594 | pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag | Express high ribosome loading circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter |
| 231595 | pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-Xbp1(S255A) | Express high ribosome loading circular RNA translation reporter 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter |
| 231596 | pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-2xXbp1(S255A) | Express high ribosome loading circular RNA translation reporter 2xALFATag-10xSunTag-2xXbp1(S255A) pause site under H1 promoter |
| 231597 | pAV-H1promoter-2xTetO-Tornado-1xALFA-5xSunTag | Express circular RNA translation reporter with 1xALFATag-5xSunTag under H1 promoter |
| 231598 | pHR-scFv-GCN4-mStayGold-GB1-dWPRE | Express scFv_GCN4-mStayGold for SunTag translation imaging |
| 231599 | pHR-ALFANb-GB1-CAAX-WPRE | Express ALFANb-CAAX |
| 231603 | pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA) | Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-4x(6xAAA) under CMV promoter |
| 231604 | pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized+8xAAA | Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-8xAAA under CMV promoter |