Genetically encoded reporters of actin filament organization in living cells and tissues
Martins CS, Iv F, Suman SK, Panagiotou TC, Sidor C, Ruso-López M, Plancke CN, Omi S, Gomes M, Llewellyn A, Bandi SR, Ramond L, Arbizzani F, Rimoli CV, Schnorrer F, Robin F, Wilde A, LeGoff L, Pedelacq J-D, Cabantous S, Rincon SA, Chandre C, Brasselet S, Mavrakis M
bioRxiv 2024.04.26.591305
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231548 | pCMVtrunc msfGFPΔC10-beta-actin | Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy |
| 231549 | pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin | Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization |
| 231550 | pCMVtrunc Affimer6-msfGFPΔN12 | Mammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy |
| 231551 | pCMVtrunc Affimer6-sfCherry2ΔN12 | Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy |
| 231552 | pCMVtrunc F-tractinN9-39-msfGFPΔN7 | Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy |
| 231555 | pCMVtrunc LifeactΔC4-msfGFPΔN7 | Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy |
| 231556 | pCMVtrunc msfGFPΔC9-LifeactΔN2 | Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy |
| 231557 | pCMVtrunc sfCherry2ΔC4-Lifeact | Mammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy |
| 231558 | pCMVtrunc msfGFPΔC10-Utr28-222 | Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy |