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An efficient and easily obtainable butelase variant for chemoenzymatic ligation and modification of peptides and proteins.

Singh AK, Antonenko A, Kocyla A, Krężel A
Microb Cell Fact. 2024 Nov 30;23(1):325. doi: 10.1186/s12934-024-02598-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
221847Butelase AYExpresses Butelase AY with Val237Ala and Thr238Tyr mutations
221848pET28-ButelaseExpresses asparaginyl endopeptidase butelase
221849eSrtA-Δ24-ASNExpresses delta 24 alpha synuclein as 6XHis-SUMO-eSrtA-LPNTG-Δ24-ASN fusion protein

Antibodies from Article