The structural basis for RNA slicing by human Argonaute2.
Mohamed AA, Wang PY, Bartel DP, Vos SM
Cell Rep. 2025 Jan 28;44(1):115166. doi: 10.1016/j.celrep.2024.115166. Epub 2024 Dec 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231371 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(D669A) | Express catalytically dead mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231372 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2 | Express human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231373 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231374 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R68A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231375 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231376 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(K98A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231377 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, K98A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231378 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97A, K98A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231379 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, R68A, R97A, K98A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231380 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97E, K98E) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231381 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231382 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H712A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231383 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A, H712A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231384 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R635A) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |
| 231385 | pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(EI 5xE) | Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells |