Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations.
Xin C, Qiao D, Wang J, Sun W, Cao Z, Lu Y, Jiang Y, Chai Y, Wang XC, Chen QJ
aBIOTECH. 2024 Mar 26;5(2):117-126. doi: 10.1007/s42994-024-00144-w. eCollection 2024 Jun.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231386 | pBG-ttLbUV2 | LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation. |
| 231387 | pAGC-Lb12-P1Bb | For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis |