ER-mitochondria distance is a critical parameter for efficient mitochondrial Ca(2+) uptake and oxidative metabolism.
Dematteis G, Tapella L, Casali C, Talmon M, Tonelli E, Reano S, Ariotti A, Pessolano E, Malecka J, Chrostek G, Kulkoviene G, Umbrasas D, Distasi C, Grilli M, Ladds G, Filigheddu N, Fresu LG, Mikoshiba K, Matute C, Ramos-Gonzalez P, Jekabsone A, Cali T, Brini M, Biggiogera M, Cavaliere F, Miggiano R, Genazzani AA, Lim D
Commun Biol. 2024 Oct 10;7(1):1294. doi: 10.1038/s42003-024-06933-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231176 | pcDNA3.1-ER-mRFP | Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cells |
| 231177 | pcDNA3.1-15nmEML-mRFP | Stabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFP |
| 231178 | pcDNA3.1-20nmEML-mRFP | Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP |
| 231179 | pcDNA3.1-30nmEML-mRFP | Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP |
| 231180 | pLV-ER-mRFP | Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, from a lentiviral vector |
| 231181 | pLV-20nmEML-mRFP | Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 20 nm from a lentiviral vector |
| 231182 | pLV-30nmEML-mRFP | Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 30 nm from a lentiviral vector |
| 231183 | pcDNA3.1-ER-EBFP2 | Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells |
| 231184 | pcDNA3.1-10nm-EBFP2 | Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nm |
| 231185 | pcDNA3.1-20nm-EBFP2 | Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nm |