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Heritable gene editing in tomato through viral delivery of isopentenyl transferase and single-guide RNAs to latent axillary meristematic cells.

Liu D, Ellison EE, Myers EA, Donahue LI, Xuan S, Swanson R, Qi S, Prichard LE, Starker CG, Voytas DF
Proc Natl Acad Sci U S A. 2024 Sep 24;121(39):e2406486121. doi: 10.1073/pnas.2406486121. Epub 2024 Sep 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
231158pEE485T-DNA encoding TRV2 with mobile gRNA targeting SlPDS
231159pEE771T-DNA encoding TRV2 with mobile gRNA targeting SlPDS
231160pDL518T-DNA encoding TRV2 with mobile gRNA targeting SlPDS
231161pDL560T-DNA encoding TRV2 with ipt and mobile gRNA targeting SlPDS
231162pDL562T-DNA encoding TRV2 with mobile gRNA targeting SlCHL1
231163pDL639T-DNA encoding TRV2 with mobile gRNAs targeting SlHAIRPLUS and SlAN2
231164pDL649T-DNA encoding TRV2 with mobile gRNA targeting SlHAIRLESS
231165pDL655T-DNA encoding gRNA targeting SlPDS
231166pDL691T-DNA encoding TRV2 with ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS
231167pEE844T-DNA encoding TRV2 with PaqCI Recognition Sites and Ccdb
231168pEE834T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Antibodies from Article