Phage-mediated intercellular CRISPRi for biocomputation in bacterial consortia.

Pujar A, Pathania A, Hopper C, Pandi A, Calderon CR, Fugger M, Nowak T, Kushwaha M
Nucleic Acids Res. 2024 Dec 27:gkae1256. doi: 10.1093/nar/gkae1256. PubMed Article

Please see (Kushwaha et al. 2017) for additional plasmids used in this article.

Plasmids from Article

ID	Plasmid	Purpose
190432	pSEVA63_Helper	M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)
190433	pSEVA63_HelperΔgIII	M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.
190434	pSB1K3_M13ps	M13 packaging signal cloned into the pSB1K3 vector.
190435	pSB1K3_M13ps_LacZα_gIII	M13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.
231407	pSEVA19_AraC_pBAD-sgRNA-1	sgRNA-1 expression from an arabinose-inducible promoter.
231408	pSEVA19_LacI_pLac-sgRNA-1	sgRNA-1 expression from an IPTG-inducible promoter.
231409	pSEVA19_sgRNA-1_M13ps	M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.
231413	pSEVA26_sgRNA-A0_M13ps	M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).
231415	pSEVA47_dCas9-GFP_AAN_4	This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.
231416	pSEVA47_dCas9-GFP_NOT_i1	This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.
231419	pSEVA47_dCas9-GFP_YES_i2	This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.