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Phage-mediated intercellular CRISPRi for biocomputation in bacterial consortia

Pujar A, Pathania A, Hopper C, Pandi A, Függer M, Nowak T, Kushwaha M
bioRxiv 2024.09.02.610857 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
190432pSEVA63_HelperM13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)
190433pSEVA63_HelperΔgIIIM13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.
190434pSB1K3_M13psM13 packaging signal cloned into the pSB1K3 vector.
190435pSB1K3_M13ps_LacZα_gIIIM13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.

Antibodies from Article