Orphan quality control shapes network dynamics and gene expression.
Mark KG, Kolla S, Aguirre JD, Garshott DM, Schmitt S, Haakonsen DL, Xu C, Kater L, Kempf G, Martinez-Gonzalez B, Akopian D, See SK, Thoma NH, Rape M
Cell. 2023 Aug 3;186(16):3460-3475.e23. doi: 10.1016/j.cell.2023.06.015. Epub 2023 Jul 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231231 | pCS2-MCRS1-GFP-IRES-mCherry | MCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression. |
| 231232 | pCS2-MYC-GFP-IRES-mCherry | MYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression. |
| 231233 | pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD) | MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells |
| 231234 | pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2) | MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cells |
| 231235 | pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD) | MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells |
| 231236 | pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD) | MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cells |
| 231237 | pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD) | MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cells |
| 231238 | pCMV 3xFLAG-UBR5 | Flag-tagged wild-type UBR5 over-expression construct for transient expression in mammalian cells |
| 231241 | pCMV 3xFLAG-MCRS1 | Flag-tagged wild-type MCRS1 over-expression construct for transient expression in mammalian cells |
| 231243 | pCS2-HA-MycΔdeg1∆deg2 | HA-tagged MYC over-expession construct with point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cells |