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John Doench Lab: Activity-based selection for enhanced base editor mutational scanning

Kaplan EG, Steger RJ, Shah ST, Drepanos LM, Griffith AL, Reint G, Doench JG
bioRxiv 2024.11.12.622254 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
231109pRDA_867A>G base editor
231111pRDB_270C>T base editor
231112pRDB_268SpG-Cas9
231113pRDB_345ABE activity-based selection cloning vector
231114pRDB_347CBE activity-based selection cloning vector
231115AA566Fragmid fragment: (guide cassette) ABE activity-based selection cloning
231116AA509Fragmid fragment: (2A Selection) ABE activity-based selection intron
231117AA568Fragmid fragment: (guide cassette) CBE activity-based selection cloning
231118AA516Fragmid fragment: (2A Selection) CBE activity-based selection intron
231119AA498Fragmid fragment: (guide cassette) ABE activity-based selection CD274 positive control
231120AA505Fragmid fragment: (guide cassette) CBE activity-based selection CD274 positive control

Antibodies from Article