Heritable, multinucleotide deletions in plants using viral delivery of a repair exonuclease and guide RNAs.
Liu D, Myers EA, Xuan S, Prichard LE, Donahue LI, Ellison EE, Starker CG, Voytas DF
Plant Physiol. 2024 Mar 29;194(4):2229-2239. doi: 10.1093/plphys/kiae015.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231145 | VirTREX2-HLDel-2_NbPDS | T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDS |
| 231146 | VirTREX2-HLDel-1_NbPDS | T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDS |
| 231147 | esgRNA_NbPDS | T-DNA encoding TRV2 with mobile gRNA targeting NbPDS |
| 231148 | VirTREX2-HLDel-1_NbSTM-5'UTR | T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbSTM-5?UTR |
| 231149 | VirTREX2-HLDel-1_NbATML1-1pro | T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbATML1-1pro |
| 231150 | esgRNA_NbSTM-5'UTR | T-DNA encoding TRV2 with mobile gRNA targeting NbSTM-5?UTR |
| 231151 | esgRNA_NbATML1-1pro | T-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1pro |
| 231152 | VirTREX2-HLDel-1_SlMIR164b | T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting SlMIR164b |
| 231153 | esgRNA_SlMIR164b | T-DNA encoding TRV2 with mobile gRNA targeting SlMIR164b |
| 231154 | VirTREX2-HLDel-1_NbATML1-2pro and NbATML1-1pro | T-DNA encoding TRV2 with TREX2 and mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro |
| 231155 | esgRNA_NbATML1-2pro and NbATML1-1pro | T-DNA encoding TRV2 with mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro |
| 231156 | VirTREX2-HLDel-1_NbmiR164e/h | T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbmiR164e/h |
| 231157 | esgRNA_NbmiR164e/h | T-DNA encoding TRV2 with mobile gRNA targeting NbmiR164e/h |