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John Ngo Lab: RNA-Stabilized Coat Proteins for Sensitive and Simultaneous Imaging of Distinct Single mRNAs in Live Cells

Kuffner CJ, Marzilli AM, Ngo JT
bioRxiv 2024.11.21.624393 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
231089pcDNA3-mNG-IRES-mScarlet-dMCPBicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.
231090pcDNA3-mNG-IRES-mScarlet-dPCPBicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.
231091minCMV-1xmNG-dMCPEncodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).
231092minCMV-1xmNG-dPCPEncodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV).
231093UBC-4xmNG-dMCPEncodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC).

Antibodies from Article