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Structural basis and regulation of the reductive stress response.

Manford AG, Mena EL, Shih KY, Gee CL, McMinimy R, Martinez-Gonzalez B, Sherriff R, Lew B, Zoltek M, Rodriguez-Perez F, Woldesenbet M, Kuriyan J, Rape M
Cell. 2021 Oct 14;184(21):5375-5390.e16. doi: 10.1016/j.cell.2021.09.002. Epub 2021 Sep 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
231001pCS2 3xFLAG FEM1ATransient overexpression of mouse FEM1A in mammalian cells
231002pCS2+ HA-BEX1Transient overexpression of human BEX1 in mammalian cells
231004pCS2+ HA BEX3Transient overexpression of human BEX3 in mammalian cells
231005pCS2+ HA-BEX4Transient overexpression of human BEX4 in mammalian cells
231007pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherryProtein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.
231008pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherryProtein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.

Antibodies from Article