A Cellular Mechanism to Detect and Alleviate Reductive Stress.

Manford AG, Rodriguez-Perez F, Shih KY, Shi Z, Berdan CA, Choe M, Titov DV, Nomura DK, Rape M
Cell. 2020 Oct 1;183(1):46-61.e21. doi: 10.1016/j.cell.2020.08.034. Epub 2020 Sep 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
230993	pCS2+ 3xFLAG-FEM1B WT	Transient overexpression of mouse FEM1B in mammalian cells
230996	pCS2+ GFP-2xESP3I-IRES-mCherry	Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.
231000	pFastBac Dual His6-TEV-CUL2, Rbx1	Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells