Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
Liu S, Hamilton MC, Cowart T, Barrera A, Bounds LR, Nelson AC, Dornbaum SF, Riley JW, Doty RW, Allen AS, Crawford GE, Majoros WH, Gersbach CA
Cell Genom. 2025 Feb 4:100766. doi: 10.1016/j.xgen.2025.100766.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
230934 | pLenti-hU6-cs1-PuroR-T2A-BFP | gRNA cloning vector with direct capture sequence 1 in stem loop 2 |