Qizhao Wang Lab: AAVone: A Cost-Effective, Single-Plasmid Solution for Efficient AAV Production with Reduced DNA Impurities
n/a
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 230928 | pAAVone-AAV9-CMV-eGFP | pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system. |
| 230929 | pAAVone-AAV-PHP.eB-CMV-eGFP | pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB serotype, utilizing the AAVone single-plasmid AAV production system. |
| 230930 | pAAVone-AAV2-CMV-eGFP | pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system. |
| 230931 | pAAVdual-CMV-eGFP | pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter. |
| 230932 | pAAVtri-CMV-eGFP | pAAVtri transgene plasmid is used for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes. |
| 230933 | mini-pHelper-1.0 | The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production |