Enhanced in vivo gene knockout with undetectable off-targets using multiplexed Cas12a sgRNAs
Port F, Buhmann MA, Zhou J, Stricker M, Vaughan-Brown A, Michalsen A-C, Roßmanith E, Pöltl A, Großkurth L, Huber J, Menendez Kury LB, Weberbauer B, Hübl M, Heigwer F, Boutros M
bioRxiv 2024.11.26.625385
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 230906 | act5C-mScarlet | Ubiquitous expression of mScarlet in Drosophila melanogaster |
| 230907 | GMR11F02-Gal4 UAS-GFP | Tissue-specific expression of GFP in wing and haltere imaginal discs. |
| 230908 | UAST-FRT-GFP-FRT-uMCas12a+nls1x | Double inducible expression of Cas12a+ with Gal4 and Flp. |
| 230909 | UAST-uMCas12a+nls2x | inducible expression of Cas12a+ with Gal4. |
| 230910 | pGRACE | Tracing of CRISPR activity in whole animals. |