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Matthew Kennedy Lab: Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors

Unpublished

Plasmids from Article

ID Plasmid Purpose
230055pAAV-hSyn-GluA1-scFv-HaloAAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.
230056pAAV-hSyn-GluA1-scFv-mNeon-KDELAAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.
230057pAAV-hSyn-GluA2-scFv-mNeon-KDELAAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

Antibodies from Article