Synthetic GPCRs for programmable sensing and control of cell behaviour.
Kalogriopoulos NA, Tei R, Yan Y, Klein PM, Ravalin M, Cai B, Soltesz I, Li Y, Ting A
Nature. 2024 Dec 4. doi: 10.1038/s41586-024-08282-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229996 | pAAV-anti-GFP(LaG2) PAGER(TF) | Expresses anti-GFP PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840) |
| 229997 | pAAV-anti-GFP(LaG17) PAGER(TF) | Expresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840) |
| 229998 | pAAV-anti-mCherry(LaM6) PAGER(TF) | Expresses anti-mCherry PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840) |
| 229999 | pAAV-anti-mCherry(LaM8) PAGER(TF) | Expresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840) |
| 230000 | pAAV-anti-VEGF(Nb35) PAGER(TF) | Expresses anti-VEGF PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840) |
| 230001 | pAAV-anti-TNFalpha(Ozoralizumab) PAGER(TF) | Expresses anti-TNFalpha PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840) |
| 230002 | pAAV-anti-CCL2(8E10) PAGER(TF) | Expresses anti-CCL2 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840) |
| 230003 | pAAV-anti-PDL1(KN035) PAGER(TF) | Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840) |
| 230004 | pAAV-UAS-mIFNγ | Gal4-dependent expression of secreted mouse IFNγ |
| 230005 | pAAV-UAS-anti-CD3/anti-CD19 BiTE | Gal4-dependent expression of secreted BiTE |
| 230006 | pAAV-anti-GFP(LaG16) PAGER(Gq) | Expresses anti-GFP PAGER(Gq) in mammalian cells |
| 230007 | pAAV-anti-GFP(LaG16) PAGER(Gs) | Expresses anti-GFP PAGER(Gs) in mammalian cells |
| 230008 | pAAV-anti-GFP(LaG16) PAGER(Gi) | Expresses anti-GFP PAGER(Gi) in mammalian cells |
| 230009 | pAAV-anti-GFP(LaG16) PAGER(G12) | Expresses anti-GFP PAGER(G12) in mammalian cells |
| 230010 | pAAV-anti-mCherry(LaM6) PAGER(Gq) | Expresses anti-mCherry PAGER(Gq) in mammalian cells |
| 230011 | pAAV-anti-mCherry(LaM6) PAGER(Gs) | Expresses anti-mCherry PAGER(Gs) in mammalian cells |
| 230012 | pAAV-anti-mCherry(LaM6) PAGER(Gi) | Expresses anti-mCherry PAGER(Gi) in mammalian cells |
| 230013 | pAAV-anti-mCherry(LaM6) PAGER(G12) | Expresses anti-mCherry PAGER(G12) in mammalian cells |
| 230014 | pAAV-anti-VEGF(Brolucizumab) PAGER(Gq) | Expresses anti-VEGF PAGER(Gq) in mammalian cell |
| 230015 | pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq) | Expresses anti-TNFalpha PAGER(Gq) in mammalian cells |
| 230016 | pCDH-anti-mCherry(LaM6) PAGER(Gq) | Lentiviral vector expressing anti-mCherry PAGER(Gq) in mammalian cells |
| 230017 | pCDH-anti-mCherry(LaM6) PAGER(Gs) | Lentiviral vector expressing anti-mCherry PAGER(Gs) in mammalian cells |
| 230018 | pCDH-anti-mCherry(LaM6) PAGER(Gi) | Lentiviral vector expressing anti-mCherry PAGER(Gi) in mammalian cells |
| 230019 | pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gi) | AAV vector expressing anti-mCherry PAGER(Gi) in neurons |
| 230020 | pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gq) | AAV vector expressing anti-mCherry PAGER(Gq) in neurons |
| 230021 | pCDH-SFFV-anti-mCherry(LaM6) PAGER(Gi) | Lentiviral vector expressing anti-mCherry PAGER(Gi) in human T cells |
| 230022 | pAAV-anti-GFP/BFP(LaG2) PAGER(FL) | Expresses anti-GFP/BFP PAGER(FL) in mammalian cells |
| 230023 | pAAV-anti-mCherry(LaM6) PAGER(FL) | Expresses anti-mCherry PAGER(FL) in mammalian cells |
| 230024 | pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL) | Expresses anti-TNFalpha PAGER(FL) in mammalian cells |
| 230025 | pAAV-anti-VEGF(Nb35) PAGER(FL) | Expresses anti-VEGF PAGER(FL) in mammalian cells |