biGMamAct: efficient CRISPR/Cas9-mediated docking of large functional DNA cargoes at the ACTB locus
Pelosse M, Marcia M
bioRxiv 2024.04.18.590029
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229954 | psLIB EF1alpha | bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning |
| 229955 | psLIB TRE | bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning |
| 229958 | psBIG1b | bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning |
| 229959 | psBIG1c | bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning |
| 229960 | psBIG1d | bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning |
| 229961 | psBIG1e | bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning |
| 229962 | psBIG2ab | bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning |
| 229963 | psBIG2abc | bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning |
| 229964 | psBIG2abcd | bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning |
| 229965 | psBIG2abcde | bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning |
| 229967 | psLIB TRE EYFP-tubulin | encodes a YFP-tubulin fusion contruct under the control of a dox inducible promoter |
| 229969 | psLIB TRE GTS-mTagBFP | encodes a mTagBFP targeted to Golgi apparatus under the control of a dox inducible promoter |
| 229971 | psBIG1a ActBT2AmcherryP2AP -TagBFPH | Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro |
| 229972 | psBIG1a ActBT2ATetP2AP - H | Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro |
| 229973 | psBIG1a ActBT2ATetP2AP - mAGH | Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro |
| 229975 | pX458 ACTB | ecnodes for spCas9 and sgRNA targeting last intron of ActinB gene |