A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans.
Khan H, Huang X, Raj V, Wang H
PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 10.1371/journal.pgen.1011541. eCollection 2025 Jan.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229866 | pHW1143 | Entry vector with FRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA in slot 4 (for SapTrap assembly) |
| 229868 | pHW622 | Entry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly) |
| 229869 | pHW1133 | Destination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCE |
| 229870 | pHW1134 | gene trap RMCE plasmid with cGAL[cGAL(DBD)-linker-QFAD]-tbb-2 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE |
| 229872 | pHW1177 | gene trap RMCE plasmid with Tet-Off[TetR-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE |
| 229873 | pHW1221 | gene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE |
| 229874 | pHW1222 | gene trap RMCE plasmid with QF2[QF(DBD)-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE |
| 229875 | pHW1223 | gene trap RMCE plasmid with LexA[LexA(DBD)-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE |
| 229876 | pHW1643 | gene trap RMCE plasmid with Tet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE |
| 229877 | pHW1391 | Negative selection marker for extrachromosomal arrays during RMCE crosses - Psnt-1::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR |
| 229878 | pHW1392 | Negative selection marker for extrachromosomal arrays during RMCE crosses- Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR |
| 229879 | pHW1385 | Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR |
| 229880 | pHW1387 | Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR |