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William Hancock Lab: DNA tensiometer reveals catch-bond detachment kinetics of kinesin-1, -2 and -3

Unpublished

Plasmids from Article

ID Plasmid Purpose
229851Kif1A-EGFP-SNAPKif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
229852Kif3A-EGFP-SNAPKif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

Antibodies from Article