Natsuko Chiba Lab: Assay for Site-specific Homologous Recombination Activity in adherent cells, suspension cells, and tumor tissues
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229846 | pCG-ACTB-C4 | Expresses wild-type Cas9 and gRNA for ACTB gene. |
| 229847 | pCG-scr | Expresses wild-type Cas9 and scrambled gRNA. |
| 229848 | pCG-NT2 | Expresses wild-type Cas9 and gRNA with non-target sequence. |
| 229849 | LentiCRISPRv2-ACTB-C4 | Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency. |
| 229850 | pBS-ACTB-C200-T7 | Donor vector for the ACTB site containing T7 sequence for detection of HR products. |