Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems
Gillespie W, Zhang Y, Ruiz OE, Cerda III J, Ortiz-Guzman J, Turner WD, Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien C-B, Kwan KM, Arenkiel BR, Devine WP, Wythe JD
bioRxiv 2024.07.13.603267
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229807 | pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410) | A CAGGS driven tet-on transactivator with an nls-EGFP reporter |
| 229808 | pB-Puro-DEST (JDW 925) | A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system |
| 229809 | pCAGEN-Dre-nls (JDW 1131) | A CAGGS driven dre recombinase followed by a c-terminal 1x nls sequence |
| 229810 | pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234) | A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction. |
| 229811 | pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316) | A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation) |
| 229812 | pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326) | A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane. |
| 229813 | pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382) | A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter. |
| 229814 | pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235) | A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction. |
| 229815 | pCAGEN-WPRE-bGH-DEST (JDW 1309) | A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts |
| 229816 | pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236) | A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction. |
| 229817 | pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002) | A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores. |
| 229819 | pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218) | A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores. |
| 229820 | pAAV-Tet-Off-FLEX-DEST (JDW 1186) | An AAV, tet-off, gateway compatible destination vector with cre dependent expression of the insert. EFS driving destablized rTA |
| 229821 | pB-DEST (JDW 1205) | A PiggyBac destination vector compatible with gateway cloning system. |
| 229822 | pGLAST-DEST (JDW 1009) | A single site gateway compatible destination vector with an upstream GLAST promotor to drive expression in glial cells. |
| 229823 | pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129) | A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling. |
| 229824 | pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970) | A PiggyBac vector with a cre dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet |
| 229825 | pB-Neo-DEST (JDW 940) | A neo selectable, PiggyBac destination vector compatible with gateway cloning system. |
| 229826 | pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721) | Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA) |
| 229828 | pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936) | A Piggybac compatible, cre dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA |
| 229829 | p5E-MCS-c-Fos-b-globin (JDW 1237) | A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron. |
| 229830 | p5E-Crestin-pro (JDW 1320) | A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression |
| 229831 | pTol2-DEST-hsp70-zCreI-BFP (JDW 1184) | A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores. |
| 229832 | pME-MCS (JDW 455) | A gateway compatible middle entry clone containing an MCS (mutiple cloning site). |
| 229833 | pXMLC2-DEST (JDW 7) | A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression. |
| 229834 | pCAGEN-DV-IRES-myr-BFP (JDW 491) | A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane |
| 229835 | pBeta-MHC-promoter DV (JDW 26) | A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression. |
| 229836 | p5E-dr-myl7-pro (JDW 1148) | A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression. |
| 229837 | pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945) | A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry. |
| 229838 | pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381) | A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator. |
| 229839 | pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130) | A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling. |
| 229840 | pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154) | A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling. |
| 229841 | pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233) | A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre |
| 229842 | pCS2-phiC31o (JDW 453) | A CMV driven Phi31 recombinase. |
| 229843 | p5E-Dll4-F2-E1B-B-Globin (JDW 1366) | A gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression. |
| 229844 | pME-StayGoldc4-Giantin (JDW 1355) | A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein |
| 229845 | pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353) | A CAGGS driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling follwed by and IRES and a myristoylated TagBFP for labeling the cell membrane. |